Transgenic mice - research update

As mentioned earlier, we have been working on identifying gene targets for soluble FGFR3 cleaved sICD fragment using ChIP-sequencing. We have made considerable progress in optimizing the protocol for this experiment and have also initiated our first ChIP-sequencing experiment. We are beginning to prepare for additional ChIP-sequencing experiments based on the results from our preliminary investigation.

Additionally, the Ach-mutant transgenic mice line is being established. We have a few different transgenic lines in our facility that are in the process of being genotyped to confirm their genetic make-up. Once confirmed, we will start using them initially to perform certain immunohistochemistry and protein studies. 

Research Update

We continue to work on ChIP-sequencing. Considering this will be the first time we will be performing a genome wide search for FGFR3 target genes, we have to do a lot of troubleshooting and run preliminary experiments to confirm our experimental conditions. We have made considerable progress and will be performing initial ChIP-sequencing experiments.

On the other hand, we are also making progress in developing transgenic mice that will be used to  discover effects of FGFR3 mutations on bone growth.