Wednesday, April 10, 2013

Transgenic mice - research update

As mentioned earlier, we have been working on identifying gene targets for soluble FGFR3 cleaved sICD fragment using ChIP-sequencing. We have made considerable progress in optimizing the protocol for this experiment and have also initiated our first ChIP-sequencing experiment. We are beginning to prepare for additional ChIP-sequencing experiments based on the results from our preliminary investigation.

Additionally, the Ach-mutant transgenic mice line is being established. We have a few different transgenic lines in our facility that are in the process of being genotyped to confirm their genetic make-up. Once confirmed, we will start using them initially to perform certain immunohistochemistry and protein studies. 

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